منابع مشابه
Isolated Chloroplasts
1. Chloroplasts prepared by the non-aqueous technique will, after fragmentation by ultrasonic treatment, incorporate [2-14C]mevalonic acid into phytoene, the first C40 compound formed in the biosynthetic sequence to coloured carotenoids. 2. With suspensions containing 3*5mg. of chlorophyll, the optimum amounts of cofactor required were ATP (10,umoles), magnesium chloride (20,umoles) and glutath...
متن کاملPhotosynthesis by isolated chloroplasts.
Photosynthesis in green plants requires not only photoelectron transport, oxygen evolution, and photophosphorylation, but also the synthetic reactions whereby carbon dioxide is assimilated and reduced to a number of organic compounds via the photosynthetic carbon reduction cycle1 and secondary biosynthetic pathways.2 Complete photosynthesis with isolated chloroplasts has for many years been a g...
متن کاملISOLATION AND CHARACTERIZATION OF CYTOCHROME b-559 FROM CHLOROPLASTS AND ETIOPLASTS OF BARLEY
A procedure permitting the isolation of large amounts of cytochrome b-559 from chloroplasts of barley is described. The isolated cytochrome b-559 has an apparent molecular weight of 120,000. The protein equivalent weight per mole of heme is 30,000. Depending on the electrophoretic conditions used, this molecule can be dissociated into components with apparent molecular weights of 31,000, 30,000...
متن کاملBiosynthesis in Isolated Acetabularia Chloroplasts
The ability of chloroplasts isolated from Acetabulana mediterranea to synthesize the protein amino acids has been investigated. When this chloroplast isolate was presented with (14)CO(2) for periods of 6-8 hr, tracer was found in essentially all amino acid species of their hydrolyzed protein Phenylalanine labeling was not detected, probably due to technical problems, and hydroxyproline labeling...
متن کاملSynthesis of Chloroplast Dna in Isolated Chloroplasts
Chloroplasts isolated from Euglena gracilis incorporated both tritiated thymidine 5'-triphosphate and tritiated deoxyadenosine 5'-triphosphate into an acid-stable fraction. The incorporation was dependent on the presence of all four deoxynucleoside triphosphates and was sensitive to treatment with deoxyribonuclease and actinomycin D. It was demonstrated that bacterial contamination could not ac...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1970
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1970.tb00279.x